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| Registros recuperados: 116 | |
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| Giardia lamblia trophozoites were incubated for 2 h with activated murine macrophages, nitric oxide (NO) donors or a superoxide anion generator (20 mU/ml xanthine oxidase plus 1 mM xanthine). Activated macrophages were cytotoxic to Giardia trophozoites (~60% dead trophozoites). This effect was inhibited (>90%) by an NO synthase inhibitor (200 µM) and unaffected by superoxide dismutase (SOD, 300 U/ml). Giardia trophozoites were killed by the NO donors, S-nitroso-acetyl-penicillamine (SNAP) and sodium nitroprusside (SNP) in a dose-dependent manner (LD50 300 and 50 µM, respectively). A dual NO-superoxide anion donor, 3-morpholino-sydnonimine hydrochloride (SIN-1), did not have a killing effect in concentrations up to 1 mM. However, when SOD (300 U/ml)... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Nitric oxide; Macrophage; Peroxynitrite; Giardia lamblia; Cytotoxicity; Superoxide. |
| Ano: 1997 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000100015 |
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| Choquet, Gwenaelle; Soudant, P; Lambert, C; Nicolas, Jean-louis; Paillard, C. |
| Vibrio tapetis is the causative agent of brown ring disease (BRD), which affects a species of clam, Ruditapes philippinarum. After incubation with V. tapetis, hemocytes lose filopods and become rounded, indicating cytotoxic activity of the bacterium. To rapidly quantify this cytotoxicity, a flow-cytometry test was developed based on the capacity of V tapetis to inhibit adhesion of clam hemocytes to plastic. Several bacteria:hemocyte ratios, the cytotoxicity of other Vibrio spp. pathogenic to bivalves, and that of various V. tapetis isolates were tested. Inhibition of adherence is detectable with as few as 5 bacteria per hemocyte. The greater cytotoxic activity of V tapetis compared to that of V splendidus and V pectenicida suggests a specific pathogenicity... |
| Tipo: Text |
Palavras-chave: Cytotoxicity; Flow cytometry; Vibrio tapetis; Ruditapes philippinarum; Bivalve hemocytes; Pathogenicity. |
| Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/00000/10761/7590.pdf |
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| Omran,M. A. A.. |
| Scorpion venom toxicity is of major concern due to its influence on human activities and public health. The cytotoxicity and apoptosis induced by scorpion L. quinquestriatus venom on two established eukaryotic cell lines (293T and C2C12) were analyzed. Both cultured cell lines were incubated with varying doses (10, 20, and 50 µg/ml) of scorpion venom in serum free medium (SFM) for 0.5, 1, 2, 4, and 8 hours at 37°C. The percentage of total lactate dehydrogenase (LDH) released in the culture during venom incubation was used as an index of cell damage. Control culture was treated with an equal amount of SFM. Cell injury was recognized morphologically and apoptosis was researched by a Fluorescing Apoptosis Detection System using the principle of TUNEL... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Scorpion venom; Leiurus quinquestriatus; Cytotoxicity; Apoptosis; LDH. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992003000200008 |
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| QUIGNARD,Etienne Louis Jacques; POHLIT,Adrian Martin; NUNOMURA,Sergio Massayoshi; PINTO,Ana Cristina da Silva; SANTOS,Elba Vieira Mustafa dos; MORAIS,Sabrina Kelly Reis de; ALECRIM,Alexandre Mascarenhas; PEDROSO,Andreza Cristiana da Silva; CYRINO,Barbara Rachel Brito; MELO,Christiane Santana de; FINNEY,Ellen Kathryn; GOMES,Erika de Oliveira; SOUZA,Katiuscia dos Santos de; OLIVEIRA,Laura Cristina Pereira de; DON,Luciana de Castro; SILVA,Luiz Francisco Rocha e; QUEIROZ,Maria Mireide Andrade; HENRIQUE,Marycleuma Campos; SANTOS,Mirian dos; PINTO,Patrícia de Souza; SILVA,Suniá Gomes. |
| 226 methanol and water extracts representing 74 mainly native plant species found in Amazonas State, Brazil, were tested at a standard concentration of 500 μg/mL for lethality towards larvae of the brine shrimp species Artemia franciscana. Several cytotoxic plant species were identified in this work: Aspidosperma marcgravianum, A. nitidum, Croton cajucara, Citrus limetta, Geissospermum argenteum, Minquartia guianensis, Piper aduncum, P. amapense, P. capitarianum, P. tuberculatum and Protium aracouchini. The results were analyzed within the context of the available traditional knowledge and uses for these plants. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Artemia franciscana; Cytotoxicity; Amazonian plants. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672003000100093 |
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| Omran,M. A. A.; Fabb,S. A.; Dickson,G.. |
| We investigated the in vitro process of cell death caused by Egyptian cobra venom on primary human embryonic kidney (293T) and mouse myoblast (C2C12) cell lines. The aim of these studies was to provide further information about triggering cell death, and suggest methods for eliminating unwanted cells, such as tumour cells. Both cell lines were treated with 10, 20, and 50 m g/ml of Egyptian cobra (Naja haje) venom in serum free media (SFM) and incubated for 8 hours. Total activities of the lactate dehydrogenase (LDH) and creatine kinase (CK) released in the culture during venom incubation were used as an indicator of the venom in vitro cytotoxicity. Cell injury was morphologically recognized and apoptosis determined by a Fluorescing Apoptosis Detection... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cobra venom; Cytotoxicity; Apoptosis. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992004000300004 |
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| Rodrigues,M.; Barbosa Junior,F.; Perussi,J.R.. |
| This paper describes the effect of dipyridamole (DIP) on the cytotoxicity of cisplatin in HEp-2 human larynx cancer cells in vitro and the nature of the interaction between cisplatin and dipyridamole. Cytotoxic assays were performed to obtain the IC50 for cisplatin. The cells were treated with 0, 20, 40, 80, 120 or 200 µM cisplatin, with or without a single concentration of DIP and incubated for 60 min at 37ºC and 5% CO2 for 3 days and then counted with a hemocytometer. The accumulation of cisplatin in the cells was measured by atomic absorption and fluorescence was used to determine the membrane binding constant of DIP. In the presence of 10, 20 and 30 µM DIP, the IC50 of cisplatin was reduced by 25, 60 and 82% in HEp-2 cells. Combination index analysis... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cisplatin; Dipyridamole; Synergism; Tumor cells; Cytotoxicity; IC50. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000400017 |
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| Mora-Christancho, J.; Zea, S.; Santos-Acevedo, M.; Newmark-Umbreit, F.. |
| La determinación de actividad antimitótica de extractos de organismos marinos genera expectativas para la obtención de sustancias con potencial anticancerígeno a partir de fuentes alternativas. Se evaluó la actividad antimitótica in vitro de extractos orgánicos crudos de 15 esponjas de la costa Caribe colombiana, en embriones del erizo de mar Lytechinus variegatus. El 80% de las especies (Spirastrella coccinea, Myrmekioderma rea, Iotrochota imminuta, Halichondria sp., Petromica ciocalyptoides, Cinachyrella kuekenthali, Biemna cribaria, Oceanapia peltata, Xestospongia proxima, Oceanapia bartschi, Polymastia tenax y Dragmacidon reticulata) mostró un nivel de actividad fuerte inhibiendo la divisiones mitóticas desde el primer estadio celular durante la... |
| Tipo: Journal Contribution |
Palavras-chave: Cytotoxicity; Cytotoxicity; Http://aims.fao.org/aos/agrovoc/c_34251. |
| Ano: 2007 |
URL: http://hdl.handle.net/1834/3472 |
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| Registros recuperados: 116 | |
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